GSM6435215: porphyromonas gingivalis W83, wild type, rep2; Porphyromo... - SRA

SRX16930623: GSM6435215: porphyromonas gingivalis W83, wild type, rep2; Porphyromonas gingivalis W83; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 12.4M spots, 3.8G bases, 1.1Gb downloads

External Id: GSM6435215_r1

Submitted by: Sichuan University

Study: Effect of deletion of trkA on gene expression of Porphyromonas gingivalis W83

PRJNA866854 • SRP390260 • All experiments • All runs

show Abstracthide Abstract

To investigate the comprehensive function of trkA in Porphyromonas gingivalis W83, we established isogenic trkA deletion strain via homologous recombination and compared the transcriptional alteration between mutant and wild type group through RNA sequencing. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Porphyromonas gingivalis W83 wild type and isogenic trkA mutant. Each group contains 3 samples.

Sample: porphyromonas gingivalis W83, wild type, rep2

SAMN30173405 • SRS14517975 • All experiments • All runs

Organism: Porphyromonas gingivalis W83

Library:

Name: GSM6435215

Instrument: HiSeq X Ten

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted from the tissue using TRIzol® Reagent according the manufacturer's instructions (Invitrogen) and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). RNA-seq transcriptome library was prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA) using 2μg of total RNA.

Runs: 1 run, 12.4M spots, 3.8G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR20911985	12,444,249	3.8G	1.1Gb	2022-11-07

ID:: 23700162

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